|From:||Dianne Holmes <firstname.lastname@example.org> (by way of histonet)|
I am cutting frozen un-fixed ferret brain at 10 microns. My problem is the sections cut clean and then as they thaw Øthey "shred" horizontallly? Looks like knife chatter but undrer the microscope , the cell population seems to be in tact . I'm at a loss!! Can anyone help?