Re: frozen ferret brain "shreds"
|From:||"A. Erickson" <firstname.lastname@example.org> (by way of histonet)|
Maybe your block is too cold, or maybe a sharper knife wit a slightlly
different angle? Freesh (unfixed) brain can be sectioned as warm as -12
to -14 C Andra Erickson
On Wed, 13 Sep 2000, Dianne Holmes wrote:
> I am cutting frozen un-fixed ferret brain at 10 microns. My problem is
>the sections cut clean and then as they thaw Øthey "shred" horizontallly?
>Looks like knife chatter but undrer the microscope , the cell
>population seems to be in tact . I'm at a loss!! Can anyone help?
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