Re: frozen ferret brain "shreds"

From:"A. Erickson" <> (by way of histonet)

Maybe your block is too cold, or maybe a sharper knife wit a slightlly
different angle?  Freesh (unfixed) brain can be sectioned as warm as -12
to -14 C  Andra Erickson

On Wed, 13 Sep 2000, Dianne Holmes wrote:

> I am cutting frozen un-fixed ferret brain at 10 microns.  My problem is
>the sections cut clean and then as they thaw Øthey "shred" horizontallly?
>Looks like knife chatter but  undrer the   microscope , the cell
>population seems to be in tact .  I'm  at a loss!!  Can anyone help?

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