Re: calretinin (by way of histonet)

We use a HIER with Calretinin.  Our protocal is drying slides for 30 min in
oven, xylene 15 min.  100% alcohol, 95% alcohol, water, into preheated
citrate buffer in steamer for 30 minutes, cool an additional 10 minutes
outside of steamer, 10  minutes in hydrogen peroxide and then load onto the
ventana automated stainer.  Our control is prostate (we use prostate chips)

Jennifer Macias, HT (ASCP)
Forum Health Western Reserve Care System
Youngstown, OH

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