Re: blueing tissue sections

From:a i d a n s c h u r r <> (by way of histonet)

I use a few drops of conc. ammonia in 200mL of water.  Works a

New Zealand

> I am a learning-by-doing-histotechnologist (I am a resident (medical
> doctor) in pathology and now have to do my research work by myself,
> because my technician is pregnant)and I need some advice:
> what can I use instead of hot running tap water for blueing the tissue
> sections after Mayer's hematoxylin? Our hot tap water is really ugly
> brown and it takes "hours" until it is clean.
> Today I tried 0.5% lithium carbonate for 30 sec. The colour is very nice,
> however I have got a lot of "arbored" precipitates (like small trees, if
> you understand what I mean) on top of the tissue, I washed and washed
> with buffer, with dist. water, with dist. water and Triton X, but I could
> not get rid of this precipitates. What is the problem with lithium
> carbonate as a blueing solution? Or may be it is the concentration?
> Any suggestions what to use instead?
> thank you for your help,
> Dr. Heike Grabsch
> Dept of Pathology
> University of Duesseldorf
> Germany

shin: device for finding furniture in the dark...
a i d a n   c   s c h u r r
     section head,  histology department
      hutt valley health
       high street
        lower hutt, new zealand

     ph.  ++64 4 5709173
     fax  ++64 4 5709214

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