Consider that the precipitate is from the Hematoxylin....Have you tried
filtering the Hematoxylin and/or getting fresh Hematoxylin?

Barb Davies
Memorial Hospital
Colorado Springs, CO



Heike Grabsch <h.grabsch@uni-koeln.de>


Heike Grabsch <h.grabsch@uni-koeln.de> on 09/12/2000 08:59:17 PM

To:   histonet@pathology.swmed.edu
cc:

Subject:  blueing tissue sections


I am a learning-by-doing-histotechnologist (I am a resident (medical
doctor) in pathology and now have to do my research work by myself,
because my technician is pregnant)and I need some advice:

what can I use instead of hot running tap water for blueing the tissue
sections after Mayer's hematoxylin? Our hot tap water is really ugly
brown and it takes "hours" until it is clean.
Today I tried 0.5% lithium carbonate for 30 sec. The colour is very nice,
however I have got a lot of "arbored" precipitates (like small trees, if
you understand what I mean) on top of the tissue, I washed and washed
with buffer, with dist. water, with dist. water and Triton X, but I could
not get rid of this precipitates. What is the problem with lithium
carbonate as a blueing solution? Or may be it is the concentration?
Any suggestions what to use instead?


thank you for your help,

Dr. Heike Grabsch
Dept of Pathology
University of Duesseldorf
Germany