Re: again blueing tissue sections
From: | Heike Grabsch <h.grabsch@uni-koeln.de> |
Geoff McAuliffe wrote:
> Perhaps the earlier proceedures are doing something that induces the ppt to
> form?
>
> 1. Run the hematoxylin proceedure by itself, no IHC or AEC. Precipitate?
> 2. Run the AEC then the hematlxylin proceedure. Precipitate?
> 3. We already know what happens when all three are run. Precipitate!
> 4. Switch to very dilute (0.025%) Thionin or Toluidine Blue for a nuclear counterstain.
>
> Geoff
Hi Geoff,
That is exactly what I plan to do on Monday. I keep you informed. thanks
for your helpful hints.
Heike
> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
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