Re: again blueing tissue sections

From:Heike Grabsch <h.grabsch@uni-koeln.de>

Geoff McAuliffe wrote:
 
> Perhaps the earlier proceedures are doing something that induces the ppt to
> form?
> 
> 1. Run the hematoxylin proceedure by itself, no IHC or AEC. Precipitate?
> 2. Run the AEC then the hematlxylin proceedure. Precipitate?
> 3. We already know what happens when all three are run. Precipitate!
> 4. Switch to very dilute (0.025%) Thionin or Toluidine Blue for a nuclear counterstain.
> 
> Geoff

Hi Geoff, 

That is exactly what I plan to do on Monday. I keep you informed. thanks 
for your helpful hints.

Heike

> --
> **********************************************
> Geoff McAuliffe, Ph.D.
> Neuroscience and Cell Biology
> Robert Wood Johnson Medical School
> 675 Hoes Lane, Piscataway, NJ 08854
> voice: (732)-235-4583; fax: -4029
> mcauliff@umdnj.edu
> **********************************************



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