|From:||"J. A. Kiernan" <firstname.lastname@example.org> (by way of histonet)|
Some of my colleagues in Biophysics measure orientation of
collagen fibres in arteries and aneurysms. They have a microscope
with a universal stage - can be rotated and tilted (3 axes
altogether) and all the angles read off from vernier scales.
Such a stage is probably expensive, but less so than a confocal
microscope. Their staining method is picro-sirius red, with
crossed polars. For cardiac muscle, shouldn't almost any
general-purpose stain be OK?
On Wed, 6 Sep 2000, Dr. Ian Montgomery. wrote:
> I want to examine the orientation of cardiac muscle fibres at the
> periphery of the left ventricle. It's handy when we carry out some
> Physiological experiments. My first thoughts are, routine H & E of serial
> sections for a couple of hundred microns. Or, thickish cryostat sections,
> stain with propidium iodide then examine using a confocal. Any ideas?
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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