Re: Renal biopsies

From:David Anderson <> (by way of histonet)

In our lab, one pathologist prefers to evaluate the renal biopsies that she
will diagnose, but all the others are evaluated by a senior
histotechnologist. We use a dissecting microscope and look for glomeruli.
They are usually not difficult to spot. Many contain blood and will appear
as small, red circles. Look at an H&E of kidney and try to imagine what the
glomeruli would look like if three dimensional. If anyone in your lab has
experience evaluating them, sit at that person's elbow and watch. Look
through the microscope and examine them yourself. After a while, you'll
begin to recognize the different structures. We take a small piece for EM,
another for immunofluorescence (frozen), and the rest for light microscopy,
trying to get at least one glom in each piece. Hope that helps.
David Anderson
Riyadh Armed Forces Hospital
Saudi Arabia

>Subject: Renal biopsies
>Date: Fri, 08 Sep 2000 20:33:30 -0400 (EDT)
>I need help.  The pathologists in our lab ask that histotechnologists
>evaluation of adequacy on renal biopsies.  I would like to know who usually
>does this function in path labs and if there are any regulations/guidelines
>from NSH or other agencies about who should perform evaluations of renal
>biopsies and what training is needed.  Also, does anyone have any tricks of
>the trade on evaluating renals.  The radiologists take the sample in
>ultrasound or CT.
>Thanks for your help in advance.

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