Re: Iron stain problem
|From:||rudy gutierrez <IMEBRUDY@inreach.com> (by way of histonet)|
Finally had a chance to respond to some of my email. Read
your inquiry on the Histonet in regards to the G. Iron Stain procedure. I
had a customer many years ago have the same problem. The problem resulted
in the customer obtaining from a tissue that was subjected to B*5 fixative
treatment prior to grossing and further processing in formalin. I also
had a customer that obtained samples from decalcified tissue.
I would strongly suggest you make sure there is no
pre-treatments in a primary fixing agent. Lastly, once in a great while
the solutions; Potassium Ferrocyanide and HCL might be from the same origin.
You very well might have to solutions that are of the same solution or a
weaker solution. Might want to just take a last review of the solutions.
Good Luck with your stain
From: Tom T. McNemar <TMcNemar@lmhealth.org>
To: Histonet (E-mail) <firstname.lastname@example.org>
Date: Wednesday, August 30, 2000 5:47 AM
Subject: Iron stain problem
>We have done Gomori's iron stain for many, many years without a problem....
>Suddenly and for no apparent reason, it just stopped working. No nothing.
>Have run a dozen different controls, changed reagents, made up new
>reagents, changed to high purity water....
>Has this happened to anyone else? It's such a simple stain. I would
>appreciate any thoughts you might have.
>Tom Mc Nemar
>Licking Memorial Hospital
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