Abizar,

You are correct about this.  However, I too was having trouble with the
plastic jars reaching the proper temperature and called Dako's technical
support.  They were the ones who instructed me to bring it up to temperature
using a microwave and then allow the slides to incubate further in the
waterbath. So if this isn't correct, we need to hear it from Dako Corp.

-Teri Johnson
Supervisor, Physicians Reference Laboratory
Overland Park, KS

----- Original Message -----
From: Abizar Lakdawalla <abizarl@innogenex.com>
To: Sebree Linda A. <la.sebree@hosp.wisc.edu>
Cc: 'Louise Taylor' <louiset@mail.saimr.wits.ac.za>; 'Histonet'
<histonet@pathology.swmed.edu>
Sent: Tuesday, September 05, 2000 2:11 PM
Subject: Re: Hercep help


> Just a comment: Changes in protocol for FDA tests are usually not allowed.
You
> have to follow the intructions to the dot.
> abizar
>
> "Sebree Linda A." wrote:
>
> > Hi Louise,
> >
> > When we use the waterbath for HIER, we first bring the buffer and slides
(in
> > a plastic coplin jar) up to a near boil in a microwave oven and then
> > transfer it to the waterbath and start our timing.  This works very well
for
> > us and is our most gentle form of HIER that we use.
> >
> > Linda A. Sebree, HT
> > University of Wisconsin Hospital & Clinics
> > Immunohistochemistry/In Situ Hybridization Laboratory
> > D4/218-2472
> > 600 Highland Avenue
> > Madison, WI  53792-2472
> >
> > (608)265-6596
> > FAX: (608)263-1568
> >
> > > -----Original Message-----
> > > From: Louise Taylor [SMTP:louiset@mail.saimr.wits.ac.za]
> > > Sent: Monday, September 04, 2000 10:08 AM
> > > To:   histonet
> > > Subject:      Hercep help
> > >
> > > HI histonetters
> > >
> > > I need some help urgently!  I am embarking on  a  hercep trial and
have
> > > run
> > > into an unexpected problem. What do you use to put your slides in to
> > > "boil"
> > > them for antigen retrieval? I tried using a glass coplin jar in my
> > > waterbath, but it shattered, and when I used a plastic one, I noticed
that
> > > the internal solution temperature did not go above 82 degrees. (I have
> > > never
> > > been required to use a waterbath for Ag retrieval, having quite
happily
> > > used
> > > a slide rack and a beaker in the microwave - but this sytem won't
really
> > > work, as it uses quite a lot of buffer).
> > >
> > > Any suggestions will be welcome, I have about 100 slides to do in the
next
> > > few days before I go on honeymoon!!
> > >
> > > Many thanks
> > >
> > > Louise Taylor
> > >
> > >
> > > Research Laboratory
> > > Department of Anatomical Pathology
> > > South African Institute for Medical Research
> > > Johannesburg
> > > South Africa
> > >
> > >
> > >
>
>