Just a comment: Changes in protocol for FDA tests are usually not allowed. You
have to follow the intructions to the dot.
abizar

"Sebree Linda A." wrote:

> Hi Louise,
>
> When we use the waterbath for HIER, we first bring the buffer and slides (in
> a plastic coplin jar) up to a near boil in a microwave oven and then
> transfer it to the waterbath and start our timing.  This works very well for
> us and is our most gentle form of HIER that we use.
>
> Linda A. Sebree, HT
> University of Wisconsin Hospital & Clinics
> Immunohistochemistry/In Situ Hybridization Laboratory
> D4/218-2472
> 600 Highland Avenue
> Madison, WI  53792-2472
>
> (608)265-6596
> FAX: (608)263-1568
>
> > -----Original Message-----
> > From: Louise Taylor [SMTP:louiset@mail.saimr.wits.ac.za]
> > Sent: Monday, September 04, 2000 10:08 AM
> > To:   histonet
> > Subject:      Hercep help
> >
> > HI histonetters
> >
> > I need some help urgently!  I am embarking on  a  hercep trial and have
> > run
> > into an unexpected problem. What do you use to put your slides in to
> > "boil"
> > them for antigen retrieval? I tried using a glass coplin jar in my
> > waterbath, but it shattered, and when I used a plastic one, I noticed that
> > the internal solution temperature did not go above 82 degrees. (I have
> > never
> > been required to use a waterbath for Ag retrieval, having quite happily
> > used
> > a slide rack and a beaker in the microwave - but this sytem won't really
> > work, as it uses quite a lot of buffer).
> >
> > Any suggestions will be welcome, I have about 100 slides to do in the next
> > few days before I go on honeymoon!!
> >
> > Many thanks
> >
> > Louise Taylor
> >
> >
> > Research Laboratory
> > Department of Anatomical Pathology
> > South African Institute for Medical Research
> > Johannesburg
> > South Africa
> >
> >
> >