Re: Help with clarification of "Lake"

From:Bryan Llewellyn <> (by way of histonet)

It usually makes very little difference whether you use a rack or a coplin
jar (or a slide mailer,  petri dish or bench top).  I have used all of these
successfully over the years.

Incidentally, you refer to a lake for *cytoplasmic* staining.  This would
likely involve basic tissue components.  Most lakes stain acid tissue
components such as nuclei (DNA).  If you have a specific method in mind,
perhaps you could mention it by name and give the procedure you are using.
There is really insufficient information to make reasoned comments.

Bryan Llewellyn

----- Original Message -----
From: <>
To: <>
Sent: September 12, 2000 4:25 PM
Subject: Help with clarification of "Lake"

> Me and my fellow coworkers are studying for our ASCP HT and we were
> if anyone out there could help us with some clarification about a lake for
> cytoplasmic staining.  We know that a Lake is the combination of a mordant
> with a dye and that the dye lake can bind to tissue components and form an
> insoluble colored deposit.  But what we would like some help with is this:
> Why is it that when you use the lake technique procedure you get different
> results from when you stain with the slide standing in a coplin jar versus
> lying flat on a stain rack?
> Sorry, since I have only gotten one reply I have reread what I wrote last
> night and would like to try asking it another way.
> Please explain to me why it is or if it is better that we place the slides
> a stain rack instead of placing it standing in a coplin jar.
> Thanks,
> Noreen Fitzgibbons
> Reno, NV
> Washoe Medical Center

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