Re: Fontana-Masson staining on appendix again

From:Bryan Llewellyn <> (by way of histonet)

Many technologists omit the gold toning for enterochromaffin staining.  Dark
brown on lighter brown is easier to see than dark grey on lighter grey.  The
toning is completely optional, as is the counterstaining with red nuclear
stains.  I often omit nuclear counterstaining.  You could also use another
part of the gut as a control.

I also suggest you stain with Lillie's modification of Schmorl's method for
reducing substances as well.  This demonstrates enterochromaffin blue-green.
The method is found in most comprehensive text books.  This will act as a
double check on the presence of the material.

The material may also be reliably demonstrated using methenamine silver
solution at 60C until sections are light-medium brown (do leave out the
chromic acid oxidation).  Continue on as with the Masson Fontana.

Bryan Llewellyn

----- Original Message -----
From: "CHEN, YUETIAN" <>
To: <>
Sent: September 13, 2000 7:56 AM
Subject: Fontana-Masson staining on appendix again

> Dear histonetters,
> A month ago, I asked a question about fixitive of Fontana-Masson staining.
> Now
> I do have a problem of this staining with appendix.  I have a very nice
> paraffin
> embeded appendix tissue and I have been doing the staining for three times
> yesterday.  I stained the tissue in silver solution until the sections
> light brown, dark brown (I did two sets).  However, after gold cloride,
> nuclear fast red staining, I did not see the staining.  My problem is that
> don't know what is the distrubution of those positive cells looks like in
> the
> appendix?  Is there a lot?  What tissues should turn black? I can not find
> pictures of Fontana-Masson staining on appendix.  Can you give me some
> suggestions?  Thank you very much.
> Yuetian Chen
> ______________________________

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