Dear Donna
I use Cyclin D1 on the Ventana and gett consistently good results, we use
Brain tissue, harsh retrieval in EDTA buffer , microwave 32 min incubation.
Tissue does not fall off. I but the antibody and the EDTA buffer from Zymed.
Anything else I can help you with, give me a call.
                                                  Dana  Dittus
                                                  Histology Supervisor
                                                  Abington Memorial Hospital
                                                   215-481-2609