We have been using Prefer fix and thinks it works quite well.

Trisha Emry
Orthodontics Research Lab

On Wed, 30 Aug 2000 HOOVER_JENNIFER@LILLY.COM wrote:

>      Dear Histonet,
>
>           I have yet another question concerning bone work. What are the
> recommended fixatives for bone tissue?  Or more specifically would anyone
> recommend 70% EtOh as a fixative? This is what we are currently using but
> with varied results. I have always preferred 10% NBF, Zinc Formalin or 4%
> Paraformaldehyde in the past but I was only working with soft tissue.  As I
> am a novice to bone histology it is rather difficult for me to justify my
> desire to change fixatives.  I do know that tissue can be stored in 70%
> EtOh after fixation. I would prefer to fix the bone first and then move to
> 70% for storage!  Any comments and/or wisdom would be greatly appreciated!
> Many thanks in advance!
>
>
> Jennifer Hoover
> Eli Lilly and  Company
>
>
>