A second reply from me Jill. Is the GABA conj with
Gluteraldehyde? If so, you should use a Glut fix and do a
pre-treatment with sod borohydride followed by periodic acid.

Good Luck!

Lorraine

On Thu, 7 Sep 2000, Jill McVee wrote:

> Thanks to Geoff,Darren,Dianne,and any one else I,ve missed or yet to
>receive..
> Diane,s answer intrigued me as I have never heard of sonicating tissue but
> I,ll give it a go. I will also try the Glutaraldehye but as I am staining
> for Gaba I wonder if this will interfer with the staining I need.Thanks
> again for the input I,ll let you know how I get on.
> Jill Mcvee
> Histologist
> St.Andrews Uni.
> Scotland.
>
>
>