Good luck doing any sort of immunochem in JB4.  The problem is that the
plastic matrix is so tightly woven that the large macro-molecules involved
in IHC have difficulty penetrating.  If you can, try persuading the
researchers to switch over to using a plastic designed for immuno.  I had
good success with Polysciences' Immunobed which is like JB4 but allows for
IHC.

*********************************
Catherine "Katie" Bresee Bennett
Sr. Research Technologist
Lovelace Respiratory Research Institute
Albuquerque, New Mexico


> -----Original Message-----
> From:	Denise Crowley [SMTP:dencrowl@MIT.EDU]
> Sent:	Thursday, September 14, 2000 11:40 AM
> To:	histonet@pathology.swmed.edu
> Subject:	staining resin embedded tissue
> 
> Hi,
> I am posting this inquiry for a postdoc working with mouse embryos.  He
> has
> some  fixed in formalin and embedded in JB4.  He would like to stain for
> fibrin.  Any suggestions?  He is also interested in doing
> immunohistochemistry for ECM (extracellular matrix) proteins.  Does anyone
> have experience with this?
> Thanks, Denise