RE: intern seeks help...
|From:||"MacDonald, Jennifer" <email@example.com> (by way of histonet)|
Did you "pretreat" your slides in Bouin's?
> From: Heather Hall[SMTP:firstname.lastname@example.org]
> Sent: Wednesday, September 13, 2000 11:20 AM
> To: email@example.com
> Subject: intern seeks help...
> My name is Heather Hall and I am presently doing an
> internship at Primedica Inc., located in Worcester,
> Mass. One of the required slides is skin stained with
> trichrome. For some reason, I cannot get this slide
> to come out right. I first tried Masson's procedure,
> with kit reagents. The slides appeared as though they
> had not been differentiated in
> phosphotungstic/phosphomolybdic acid long enough, as
> the collagen bundles were retaining the scarlet-acid
> fuchsin in their centers. My first step to correct
> the problem was to increase the time in the p/p acid;
> that didn't work. Next I made up fresh reagents;
> didn't work. Altering the times in the other reagents
> didn't work either, so I tried the Gomori's procedure.
> When the Gomori's did not work, I changed species
> (first species used was pig, second was human). The
> same problem was occuring in the human tissue also.
> Since the control slides appeared to coming out fine I
> tried a third species. I tried rat skin hoping that
> it might have a thinner connective tissue layer and
> permeability would be altered, but that came out as
> badly as the others. I don't know what to do next to
> try and fix this problem. Any suggestions would be
> very much appreciated. Thanks for any help you may be
> able to give.
> Heather Hall
> Primedica Inc.
> Histology Lab
> Worcester, MA
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