RE: frozen ferret brain "shreds"
|From:||Alan Bright <email@example.com>|
looks like you are sectioning the ferret brain at to cold a temperature, try
to section at -8 to -12 degs.C. also try to cool the knife and anti-roll
plate to around -20 deg.C. This will solve your problem, if not also look at
Bright Instrument Co.Ltd.
St Margaret's Way
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Web Site: www.brightinstruments.com
From: Dianne Holmes [mailto:firstname.lastname@example.org]
Sent: 13 September 2000 16:56
Subject: frozen ferret brain "shreds"
I am cutting frozen un-fixed ferret brain at 10 microns. My problem is the
sections cut clean and then as they thaw Othey "shred" horizontallly?
Looks like knife chatter but undrer the microscope , the cell population
seems to be in tact . I'm at a loss!! Can anyone help?
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