Dear Dianne,

looks like you are sectioning the ferret brain at to cold a temperature, try
to section at -8 to -12 degs.C. also try to cool the knife and anti-roll
plate to around -20 deg.C. This will solve your problem, if not also look at
knife sharpness.

Best Regards

Alan Bright

Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
PE18 6EB
England

Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: AlanBright@brightinstruments.com
Web Site: www.brightinstruments.com


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-----Original Message-----
From: Dianne Holmes [mailto:dholmes@anatomy.umsmed.edu]
Sent: 13 September 2000 16:56
To: histonet@pathology.swmed.edu
Subject: frozen ferret brain "shreds"


I am cutting frozen un-fixed ferret brain at 10 microns.  My problem is the
sections cut clean and then as they thaw Othey "shred" horizontallly?
Looks like knife chatter but  undrer the   microscope , the cell population
seems to be in tact .  I'm  at a loss!!  Can anyone help?