RE: Skeletal Preps

From:Bert Dotson <> (by way of histonet)

I think the method you are referring to is found in:
Dingerkus, G. and Uhler, L. "Enzyme Clearing of Alcian Blue Stained Whole
Small Vertebrates for Demonstration of Cartilage" Stain Technology V52 N.4
July 1997 p. 229-232 The technique uses alizarin red for the bone, alcian
blue for cartilage and a trypsin-KOH sequence to clear the tissue. If you
can't locate the article I could have it copied and faxed but B&W won't do
the absolutely beautiful pictures justice.

Bert Dotson

-----Original Message-----
From:	Robin E. Taylor []
Sent:	Wednesday, September 06, 2000 9:12 AM
To:	histonet
Subject:	Skeletal Preps

Does anyone have a procedure for doing skeletal preps (mouse) using alcian
and alizarin red?



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