RE: Hercep help

From:"Sebree Linda A." <> (by way of histonet)

Hi Louise,

When we use the waterbath for HIER, we first bring the buffer and slides (in
a plastic coplin jar) up to a near boil in a microwave oven and then
transfer it to the waterbath and start our timing.  This works very well for
us and is our most gentle form of HIER that we use.

Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
600 Highland Avenue
Madison, WI  53792-2472

FAX: (608)263-1568

> -----Original Message-----
> From:	Louise Taylor []
> Sent:	Monday, September 04, 2000 10:08 AM
> To:	histonet
> Subject:	Hercep help
> HI histonetters
> I need some help urgently!  I am embarking on  a  hercep trial and have
> run
> into an unexpected problem. What do you use to put your slides in to
> "boil"
> them for antigen retrieval? I tried using a glass coplin jar in my
> waterbath, but it shattered, and when I used a plastic one, I noticed that
> the internal solution temperature did not go above 82 degrees. (I have
> never
> been required to use a waterbath for Ag retrieval, having quite happily
> used
> a slide rack and a beaker in the microwave - but this sytem won't really
> work, as it uses quite a lot of buffer).
> Any suggestions will be welcome, I have about 100 slides to do in the next
> few days before I go on honeymoon!!
> Many thanks
> Louise Taylor
> Research Laboratory
> Department of Anatomical Pathology
> South African Institute for Medical Research
> Johannesburg
> South Africa

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