RE: Fontana-Masson staining on appendix again
|From:||Ballnclaw@aol.com (by way of histonet)|
Enterochromaffin or argentaffin cells are small ovoid cells and they are most
abundant in the antrum (pyloric gland region) where they secrete the hormone
gastrin. They contain small, scattered dense secretory granules. Besides the
stomach, these cells are found throughout the G.I. tract, more commonly in
the duodenum. They are located among epithelial cells lining the crypts of
Lieberkühn (intestinal glands) as cells with fine granules that stain
brown/black with silver salts (argentophilic), during the Fontana-Masson
technique. They are exactly located in the abluminal portion of the cell
between the nucleus and the basement membrane.
Check your slides after a full hour in solution at 56C for proper brown/black
staining intesity of these granules.
The vermiform appendix is nearly identical to the colon except it is small,
has semi-confluent lymphatic nodules, and the epithelium is mostly goblet
cells with fewer argentaffin cells than the small intestine. It would be
preferable (if available) to use duodenum or jejunum as a positive control
for the Fontana-Masson technique in the future.
Eric C. Kellar
University of Pittsburgh Medical Center
A month ago, I asked a question about fixitive of Fontana-Masson staining.
I do have a problem of this staining with appendix. I have a very nice
embeded appendix tissue and I have been doing the staining for three times
yesterday. I stained the tissue in silver solution until the sections turn
light brown, dark brown (I did two sets). However, after gold cloride, and
nuclear fast red staining, I did not see the staining. My problem is that I
don't know what is the distrubution of those positive cells looks like in
appendix? Is there a lot? What tissues should turn black? I can not find
pictures of Fontana-Masson staining on appendix. Can you give me some
suggestions? Thank you very much.
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