Louise,
I have had some antibodies completely lose antigenicity after holding
overnight, and some have had no problems at all.  It will simply depend on
the ab.  CD markers are the worst in my situation.  PanKeratin and S-100
lose the least
Wanda Shotsberger
jefwan@infoave.net

-----Original Message-----
From: Louise Taylor [mailto:louiset@mail.saimr.wits.ac.za]
Sent: Tuesday, August 29, 2000 6:43 AM
To: histonet
Subject: Dewaxing slides


Hi histonetters

Has anyone had any experience with loss of antigenicity in slides that have
been dewaxed and either left soaking in water/buffer overnight or allowed to
airdry?

The reason I ask this is that we are moving to an automated IHC sytem in the
near future and will be dealing with a lot of slides and a short turn around
time.

Any input appreciated

Louise Taylor
Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa