Proteolytic enzymes

From:Nima Farsinejad <> (by way of histonet)

	I have noticed that most antigen retrieval protocols that were
sent to me (and many thanks to everyone that did!) included some type of
heat treatment. However I want to perform single cell gene array studies
and I do not wish to heat the tissue, therefore I am thinking about using
a proteolytic enzyme instead (Proteinase K, Pepsin, pronase, or trypsin).
However, before I do this, I want to ask the very smart people in Histonet
:o) about your opinion on using this enzymes and the possibility of some
other technique that might compensate the antigen masking produced by
formaldehyde fixation of paraffin embedded tissue.


Nima Farsinejad
Research Specialist
Digestive Diseases
Emory University
Atlanta, Georgia

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