|From:||Nima Farsinejad <firstname.lastname@example.org> (by way of histonet)|
I have noticed that most antigen retrieval protocols that were
sent to me (and many thanks to everyone that did!) included some type of
heat treatment. However I want to perform single cell gene array studies
and I do not wish to heat the tissue, therefore I am thinking about using
a proteolytic enzyme instead (Proteinase K, Pepsin, pronase, or trypsin).
However, before I do this, I want to ask the very smart people in Histonet
:o) about your opinion on using this enzymes and the possibility of some
other technique that might compensate the antigen masking produced by
formaldehyde fixation of paraffin embedded tissue.
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