May-Grunwald Giemsa

From:rzumbo <> (by way of histonet)

HI all,
A colleague is having trouble with a May-Grunwald-Giemsa stain.
She is doing the stain on formalin fixed tissue mainly hearts, some
of which are 2 years old. She is doing a modified May-Grumwald-
Giemsa ( Manual of Histological Techniques and their Diagnostic
Application,Bancroft & Cook pg 328) using Sorensons buffer. The
section after differentiation are perfect. After drying the sections
have gone bright blue and the features we are looking for (mainly
Eosinophils and mast cells) are all blue. Does anyone know what
is causing it and how it can be fixed.

Rosalba Zumbo
Technical Officer ( Scientific)
Institute of Forensic Medicine
Sydney, Australia

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