ISH/IF help needed

From:Tamara Howard <> (by way of histonet)

Please excuse me if you've already received this message - I sent it
yesterday to both servers, but received it from neither, so I think our
system ate it on its way out :)


---------- Forwarded message ----------
Date: Wed, 6 Sep 2000 10:22:45 -0400 (EDT)
From: Tamara Howard <>
To: Microscopy Listserver <>,
     Histology listserver <>
Subject: Bio:sample storage/transport


A researcher here has some cells (on slides) that were fixed in
methanol/acetic acid and then stored in the meth/acetic at -20C. Problem
is, the slides are in Europe and we are trapped on Long Island, NY, USA;
but we need the slides here. Sigh.

Any ideas as to the best way to have them shipped? Should we:

a) Leave them in the fix and shipped on ice (dry or wet)

b) Leave them in the fix and shipped at ambient temperature

c) Have them dehydrated and sent dry at ambient

d) something else? (I'm thinking sacrifice a chicken to the gods of

Once they get here, they will be immunofluoresced and DNA FISHed, if that
makes any difference.

As usual, these are *very* valuable, non-replaceable samples so we don't
have the capability of trying various procedures...we're hoping to get a
"we've done this and it works" response.

Thank you SO much!

Tamara Howard

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