IHC on Mouse cell cultures

From:Colleen Forster <cforster@tc.umn.edu> (by way of histonet)

Hello Histonetters,

I am trying to do IHC on mouse CNS cell culture slides. If I
do monoclonal antibodies the results are great! However, when I use
polyclonals either goat or rabbit the bkgd is exceptionally high.
Could some of you please share some suggestions on cleaning up
this problem??

As always, Thanks in advance.

-- Colleen Forster
U of MN, Dept. of Neurology

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