HI histonetters

I need some help urgently!  I am embarking on  a  hercep trial and have run
into an unexpected problem. What do you use to put your slides in to "boil"
them for antigen retrieval? I tried using a glass coplin jar in my
waterbath, but it shattered, and when I used a plastic one, I noticed that
the internal solution temperature did not go above 82 degrees. (I have never
been required to use a waterbath for Ag retrieval, having quite happily used
a slide rack and a beaker in the microwave - but this sytem won't really
work, as it uses quite a lot of buffer).

Any suggestions will be welcome, I have about 100 slides to do in the next
few days before I go on honeymoon!!

Many thanks

Louise Taylor


Research Laboratory
Department of Anatomical Pathology
South African Institute for Medical Research
Johannesburg
South Africa