|From:||Louise Taylor <firstname.lastname@example.org> (by way of histonet)|
I need some help urgently! I am embarking on a hercep trial and have run
into an unexpected problem. What do you use to put your slides in to "boil"
them for antigen retrieval? I tried using a glass coplin jar in my
waterbath, but it shattered, and when I used a plastic one, I noticed that
the internal solution temperature did not go above 82 degrees. (I have never
been required to use a waterbath for Ag retrieval, having quite happily used
a slide rack and a beaker in the microwave - but this sytem won't really
work, as it uses quite a lot of buffer).
Any suggestions will be welcome, I have about 100 slides to do in the next
few days before I go on honeymoon!!
Department of Anatomical Pathology
South African Institute for Medical Research
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