Me and my fellow coworkers are studying for our ASCP HT and we were wondering

if anyone out there could help us with some clarification about a lake for
cytoplasmic staining.  We know that a Lake is the combination of a mordant
with a dye and that the dye lake can bind to tissue components and form an
insoluble colored deposit.  But what we would like some help with is this:
Why is it that when you use the lake technique procedure you get different
results from when you stain with the slide standing in a coplin jar versus
lying flat on a stain rack?

Sorry, since I have only gotten one reply I have reread what I wrote last
night and would like to try asking it another way.
Please explain to me why it is or if it is better that we place the slides on
a stain rack instead of placing it standing in a coplin jar.

Thanks,

Noreen Fitzgibbons
Reno, NV
Washoe Medical Center