Fwd: intern seeks help...
|From:||"Dr. Ian Montgomery." <email@example.com> (by way of histonet)|
Spell it out. What fixative, nuclear stain and your recipe for the
ponceau de xyledene and light green. Have you also thought of a Mallory,
lovely stain and looks good on skin.
>Date: Wed, 13 Sep 2000 09:20:25 -0700 (PDT)
>From: Heather Hall <firstname.lastname@example.org>
>Subject: intern seeks help...
>My name is Heather Hall and I am presently doing an
>internship at Primedica Inc., located in Worcester,
>Mass. One of the required slides is skin stained with
>trichrome. For some reason, I cannot get this slide
>to come out right. I first tried Masson's procedure,
>with kit reagents. The slides appeared as though they
>had not been differentiated in
>phosphotungstic/phosphomolybdic acid long enough, as
>the collagen bundles were retaining the scarlet-acid
>fuchsin in their centers. My first step to correct
>the problem was to increase the time in the p/p acid;
>that didn't work. Next I made up fresh reagents;
>didn't work. Altering the times in the other reagents
>didn't work either, so I tried the Gomori's procedure.
> When the Gomori's did not work, I changed species
>(first species used was pig, second was human). The
>same problem was occuring in the human tissue also.
>Since the control slides appeared to coming out fine I
>tried a third species. I tried rat skin hoping that
>it might have a thinner connective tissue layer and
>permeability would be altered, but that came out as
>badly as the others. I don't know what to do next to
>try and fix this problem. Any suggestions would be
>very much appreciated. Thanks for any help you may be
>able to give.
>Do You Yahoo!?
>Yahoo! Mail - Free email you can access from anywhere!
Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
Tel: 0141 339 8855. Extn:6602.
Fax: 0141 330 2923
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