Fwd: intern seeks help...

From:"Dr. Ian Montgomery." <ian.montgomery@bio.gla.ac.uk> (by way of histonet)

         Spell it out. What fixative, nuclear stain and your recipe for the
ponceau de xyledene and light green. Have you also thought of a Mallory,
lovely stain and looks good on skin.

>Date: Wed, 13 Sep 2000 09:20:25 -0700 (PDT)
>From: Heather Hall <hallhk@yahoo.com>
>Subject: intern seeks help...
>To: histonet@pathology.swmed.edu
>My name is Heather Hall and I am presently doing an
>internship at Primedica Inc., located in Worcester,
>Mass.  One of the required slides is skin stained with
>trichrome.  For some reason, I cannot get this slide
>to come out right.  I first tried Masson's procedure,
>with kit reagents.  The slides appeared as though they
>had not been differentiated in
>phosphotungstic/phosphomolybdic acid long enough, as
>the collagen bundles were retaining the scarlet-acid
>fuchsin in their centers.  My first step to correct
>the problem was to increase the time in the p/p acid;
>that didn't work.  Next I made up fresh reagents;
>didn't work.  Altering the times in the other reagents
>didn't work either, so I tried the Gomori's procedure.
>  When the Gomori's did not work, I changed species
>(first species used was pig, second was human).  The
>same problem was occuring in the human tissue also.
>Since the control slides appeared to coming out fine I
>tried a third species.  I tried rat skin hoping that
>it might have a thinner connective tissue layer and
>permeability would be altered, but that came out as
>badly as the others.  I don't know what to do next to
>try and fix this problem.  Any suggestions would be
>very much appreciated.  Thanks for any help you may be
>able to give.
>Heather Hall
>Primedica Inc.
>Histology Lab
>Worcester, MA
>Do You Yahoo!?
>Yahoo! Mail - Free email you can access from anywhere!

Dr. Ian Montgomery,
West Medical Building,
University of Glasgow,
G12 8QQ.
Tel: 0141 339 8855.  Extn:6602.
Fax: 0141 330 2923
e-mail: ian.montgomery@bio.gla.ac.uk

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