Fontana-Masson staining on appendix again
From: | "CHEN, YUETIAN" <YUETIAN.CHEN@spcorp.com> (by way of histonet) |
Dear histonetters,
A month ago, I asked a question about fixitive of Fontana-Masson staining.
Now
I do have a problem of this staining with appendix. I have a very nice
paraffin
embeded appendix tissue and I have been doing the staining for three times
yesterday. I stained the tissue in silver solution until the sections turn
light brown, dark brown (I did two sets). However, after gold cloride, and
nuclear fast red staining, I did not see the staining. My problem is that I
don't know what is the distrubution of those positive cells looks like in
the
appendix? Is there a lot? What tissues should turn black? I can not find
pictures of Fontana-Masson staining on appendix. Can you give me some
suggestions? Thank you very much.
Yuetian Chen
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