formalin pigment removal

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From:"Badley, Carmen M SrA LRMC" <Carmen.Badley@lnd.amedd.army.mil>
To:"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>
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Date:Mon, 25 Oct 1999 09:07:10 +0200
Content-Type:text/plain; charset="iso-8859-1"

I am currently working on my certification (HT) and have ran into a problem.
I am performing an iron stain on spleen that is full of formalin pigment.  I
do not have picric acid to remove the formalin pigment so I tried Bouin's,
which worked great.  The only problem is that the glacial acetic acid is
wiping out the iron.  Does anyone have any suggestions to help me save the
iron while removing the formalin pigment???  thank you!



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