Trypsin Digestion
<< Previous Message | Next Message >>
| From: | Ian Montgomery <ian.montgomery@bio.gla.ac.uk> (by way of histonet) |
| To: | histonet@histosearch.com |
| Reply-To: | |
| Date: | Mon, 31 Jan 2000 22:47:06 -0500 |
| Content-Type: | text/plain; charset="us-ascii" |
>Date: Fri, 29 Oct 1999 11:27:55 -0400
>From: "Bourassa, Patricia" <patricia_bourassa@groton.pfizer.com>
>Subject: Trypsin Digestion
>To: "Histonet @Pathology.Swmed.Edu (E-mail)" <histonet@pathology.swmed.edu>
>
>Hi, all!
>
>When digesting with Trypsin for Immuno, what is the common practice? Do you
>buy a "kit" or make it up fresh with a solid. And what vendors are
>recommended? Thanks!
>
>-Patti Bourassa
> Pfizer, Groton
>
Patti,
I normally use 0.1% trypsin in 0.1% Calcium chloride, ph7.8. The
trysin of
choice is from ICN Cat No: 150213
Ian
<< Previous Message | Next Message >>