Re: warm water block soak
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From: | Mary Stevens <mstevens@genetics.com> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Date: | Mon, 31 Jan 2000 22:47:19 -0500 |
Content-Type: | text/plain; charset="us-ascii" |
Hi Maureen,
I learned a simialr trick 19 years ago in my first job, from a gentlemen
who was about ready to retire at the time. He took the idea one step
further and added hand soap. A few squirts into luke warm water did
wonders (followed by brief icing).
Thanks for bringing this back around!
Mary
>>> <NEVADUNNE@aol.com> - 10/27/1999 8:16 PM >>>
Hi everyone,
I'm an old war-horse HT working in a small community hospital doing
routine histology. I'm just curious if anyone else has used a warm water soak
for their paraffin blocks (after facing) to improve sectioning. I have found
this technique very helpful in the sectioning of brittle biopsies and bloody
specimens. The crushed ice and water soak prior to sectioning allows a few
good sections after soak, but the warm water soak lets us get several ribbons
before we encounter dried out tissue. This has cut down on chatter, time
spent re-soaking and over-all quality of sections.
Any feedback?
Maureen Tomblin HT(ASCP)
Union Hospital
Elkton, MD
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