Re: rapid AE1/AE3

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From:Tony Henwood <>, (Marija Vaicaitis)
Date:Mon, 25 Oct 1999 20:50:10 +0000
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Dear Marija,

> We routinely do an AE1/AE3 on all sentinel nodes. But now the question came up-if there is a possibility of doing a rapid
> AE1/AE3 on frozen section slides.   I would appreciate any information!
It is definitely possible.
I developed a rapid IPX technique for P. carinii using a routine IPX 
method and the Lipshaw Stainplate. Incubations were done at 42oC and 
total time was decreased form 2.5 hours to 34 minutes (Aust J  Med 
Lab Sc 12:59-61, 1991).

By using direct labelling techniques, or one of the Commercial rapid 
antibody techniques (eg DAKO's) as well as 42oC incubations, it would 
be possible to obtain a result in 10 minutes. Remember, if you fix 
the frozen sections in alcohol, you won't need to antigen retrieve 
for AE1/AE3.

I hope this helps.

Rgards, Tony.

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital

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