Dear Marija,

> We routinely do an AE1/AE3 on all sentinel nodes. But now the question came up-if there is a possibility of doing a rapid
> AE1/AE3 on frozen section slides.   I would appreciate any information!
> 
It is definitely possible.
I developed a rapid IPX technique for P. carinii using a routine IPX 
method and the Lipshaw Stainplate. Incubations were done at 42oC and 
total time was decreased form 2.5 hours to 34 minutes (Aust J  Med 
Lab Sc 12:59-61, 1991).

By using direct labelling techniques, or one of the Commercial rapid 
antibody techniques (eg DAKO's) as well as 42oC incubations, it would 
be possible to obtain a result in 10 minutes. Remember, if you fix 
the frozen sections in alcohol, you won't need to antigen retrieve 
for AE1/AE3.

I hope this helps.

Rgards, Tony.
.

Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA

http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html