Dear Histonneters, We have a student doing her PhD studying CYPRIDS. A Cyprid
is the free swimming stage of the Sea - Water Barnacles before it settles
permanently! Our student Marsha would like to look @ the lipid content/volume
& also the protein content/volume of individual Cyprids. Marsha also wants to
look @ the nervous system Histologically to see if it changes morphologically
as they age/mature in the Cyprid stage.....as well as the  newly settled
Barnacle stage. What fixative would be used (15% Formalin made up in
SeaH2o...no buffer?) & is it possible to demonstrate both lipids & proteins
with the same procedure/method etc. Marsha also has access to a Confocal
Microscope(eg. can she look @ the specimen under the Confocal & say it has
X-amount of protein & X-amount of Lipid??) ANY help/suggestions will be
greatly appreciated.......................
Cheers, Bruce (& Marsha) in OZ.


--
BRUCE ABALOZ
HISTOLOGIST
DEPARTMENT of ZOOLOGY	         *  ph:    +61 3 93446282
The UNIVERSITY of MELBOURNE      *  fax:   +61 3 93447909
Parkville Victoria.3052          *  email: b.abaloz@zoology.unimelb.edu.au

AUSTRALIA.
                    IF YOU WANT TO BE HAPPY......BE!!
                    I DO NOT SEEK............I FIND!!

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