Re: Lipids In Barnacle Cyprids!!

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From:Bruce Abaloz <> (by way of histonet)
Date:Mon, 31 Jan 2000 22:47:13 -0500
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Dear Histonneters, We have a student doing her PhD studying CYPRIDS. A Cyprid
is the free swimming stage of the Sea - Water Barnacles before it settles
permanently! Our student Marsha would like to look @ the lipid content/volume
& also the protein content/volume of individual Cyprids. Marsha also wants to
look @ the nervous system Histologically to see if it changes morphologically
as they age/mature in the Cyprid well as the  newly settled
Barnacle stage. What fixative would be used (15% Formalin made up in buffer?) & is it possible to demonstrate both lipids & proteins
with the same procedure/method etc. Marsha also has access to a Confocal
Microscope(eg. can she look @ the specimen under the Confocal & say it has
X-amount of protein & X-amount of Lipid??) ANY help/suggestions will be
greatly appreciated.......................
Cheers, Bruce (& Marsha) in OZ.

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