On Wed, 27 Oct 1999, John Lydon wrote:

> One of my scientist's needs to use Kahle's fixative for an experiment.
> Anyone heard of it?

    Water:        150 ml
    95% alcohol:   65 ml
    Formalin:      30 ml
    Acetic acid:   25 ml

   From Peter Gray's 1954 "Microtomist's Formulary and Guide" p. 191.
   The ref cited there is Kahle 1908: Zoologica (Originalabhandlungen.
   Herausgegeben von C. Chun, Stuttgart) 21, 10.

   It looks like another low-alcohol AFA, quite closely similar to
   Davidson's fixative, which is a subject of HistoNet messages
   from time to time.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1