Re: Aqueous mounting media

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From:"J. A. Kiernan" <>
To:greg tesdall <>
Date:Tue, 26 Oct 1999 00:04:54 -0400 (EDT)
Content-Type:TEXT/PLAIN; charset=US-ASCII

On Mon, 25 Oct 1999, greg tesdall wrote:

> aqueous mounting media.  I do not have any. Is there
> something I could use (from the kitchen)

  Good for you! Use a drop of glycerol (= glycerin)
  and seal the edges of the coverslip with nail varnish.
  If you have no glycerol, corn syrup will do. If 
  the slides have to be kept for years, you'll need to
  mix something that sets by itself and contains an
  antibacterial preservative. For lipid stains (oil
  red O, Sudan black B etc) the pH doesn't matter.
  For most other purposes (immunofluorescence, alum-
  heamatoxylin, basic dyes) it should be above 7.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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