Re: Animal Tissue

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From:Jeff Crews <> (by way of histonet)
Date:Mon, 31 Jan 2000 22:47:29 -0500
Content-Type:text/plain; charset="us-ascii"

     A few things, probably obvious, about animal skin: Mouse skin will
     roll up like a scroll during fixation if you don't adhere it to a
     piece of card before dropping it into fix. The subQ is sticky enough
     to adhere it, and any cellulose fibers sticking to the bottom after
     processing don't cause any concern as long as everyone knows what they
     Second, most animal hair, especially hog bristles, are much harder
     than human hair, so proper embedding is even more important. Thick
     rabbit fur will trap air bubbles and make for a poor-looking ribbon,
     but it doesn't actually affect the quality of the section.
                                                        jeff C

______________________________ Reply Separator
Subject: Animal Tissue
Author:  Beverly Robinson <> at internet
Date:    10/26/1999 5:04 PM


Hi! Haven't been on board for awhile, but now I have a
broad question for all of you in vet or research, or
any lab that deals with animal tissue. I've been asked
to give a workshop on this subject. Please share with
me some examples of how animal tissue differs from
human tissue? And how did you deal with the difficult
tissues presented to you? This workshop will be mostly
basic procedures with maybe a little immuno thrown in.
One thing I've learned from being an HT for 26 years
is that there is so much more out there that I can
learn from all of you. I don't want to present a
narrow view at this workshop.
I've worked at the University of Florida vet school
and also in a large research facility dealing with
large numbers of animal tissue for a total of 10 years
experience in the animal tissue field.
Thank you so much for your imput in advance :-)

Bev Robinson HT(ASCP)
Histology Tech. Services -Self employed - Part time
Richard-Allan Scientific - Part time in Tech-Rite
Kalamazoo, MI

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