RE: sucrose/OCT
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| From: | Cynthia Favara <cfavara@niaid.nih.gov> (by way of histonet) |
| To: | histonet@histosearch.com |
| Reply-To: | |
| Date: | Mon, 31 Jan 2000 22:47:10 -0500 |
| Content-Type: | text/plain; charset="us-ascii" |
Denise,
I work with mouse tissue that has been fixed for 12-24 hrs. Place in
25% sucrose wt/vol overnight. Make sure the specimen sinks to the bottom.
Remove blot gently and sanp freeze in OCT. Good Luck!
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
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