RE: histonet button
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From: | "Culberson, Cathy" <CCulberson@carolinas.org> |
To: | 'HistoNet Server' <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Fri, 22 Oct 1999 07:18:50 -0400 |
Content-Type: | text/plain; charset="iso-8859-1" |
Please send a button this way as we were not allowed to travel.
Thanks so much.
Cathy
Cathy Culberson, MS
Supervisor, General Surgery Research
(704)355-2605
Email:cculberson@carolinas.org
-----Original Message-----
From: HistoNet Server [SMTP:histonet@pathology.swmed.edu]
Sent: Friday, October 22, 1999 1:01 AM
To: HistoNet Server
Subject: Daily Digest
----------------------------------------------------------------------
Date: 21 Oct 1999 00:09:41 -0500
From: Kathy Gorham <kathyg@eoni.com>
Subject: staffing survey
Hi, I'm working on my budget for next year and would like to show a
justification for an increase in staffing. If you could take the
time to
fill out this survey I would greatly appreciate it.
Processing #per year
Blocks
Slides
Frozen
Special Stains
Immuno
Patients
Staffing # hrs per day
Technologist
Assistance
Others
Do you assist/provide the following Services
Coding
Frozen Sections
Autopsies
Billing data entry
Grossing
Slide/Block Filing
Thanks, Kathy Gorham, HT
Grande Ronde Hospital
LaGrande, Or. 97850
----------------------------------------------------------------------
Date: 21 Oct 1999 02:30:39 -0500
From: "Jim Manavis" <jim.manavis@imvs.sa.gov.au>
Subject: Progesterone receptor
Can any one provide me with information on what Progesterone
receptor clones
they are using. Which is the clone of choice and why?
We currently use an Abbott rat anti human (clone KD68) on our FFPE
breast
material, which we enhance. Apart from this we get a very good
result.
I am currently assessing the Dako mouse monoclonal PgR (clone PgR
636). The
staining pattern looks very similar, but with a much more florid
intensity
and at a much better dilution.
If PgR 636 is the marker of choice could someone supply me with the
appropriate references/verification.
Thanks
Jim
e-mail: jim.manavis@imvs.sa.gov.au
or via Histonet
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Date: 21 Oct 1999 02:31:03 -0500
From: "Sarah A. Jones" <hawkmoon15@earthlink.net>
Subject: Histonet Buttons
Once again I was chained to my friendly exhibitor's booth, and was
unable to
even get upstairs to get a Histonet Button! In fact I didn't even
know
where to find them until Monday, when I finally discovered someone
who knew
where to find them just before I had to leave for the airport!!
So....if you would kindly consider sending one to me I would really
appreciate it!
Sarah A. Jones
540 N. Santa Cruz Ave.
PMB #297
Los Gatos, CA 95030
----------------------------------------------------------------------
Date: 21 Oct 1999 08:45:21 -0500
From: Cynthia Favara <cfavara@niaid.nih.gov>
Subject: RE: antibody to EBV antigen LMP-2
Sarka,
The only antibody I know of with those initials is Lysosomal
Marker
Protien and they are numbered, don't know of any connection with EBV
Cynthia Favara
NIAID/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
PH: 406-363-9317
FAX: 406-363-9286
----------------------------------------------------------------------
Date: 21 Oct 1999 08:45:46 -0500
From: "Tony Henwood" <henwood@mail.one.net.au>
Subject: Re: IF
Dear Sally,
> Has anyone ever performed IF on formalin fixed paraffin embedded
tissue? If
> yes. Is there an enzyme digesting agent that is better to use for
IF? Also I
> think I read somewhere that 10% Neutral Buffered Formalin
autofluoresces.
> Has anyone heard this?
You can do IF on formalin fixed tissue just like a regular IPX (it
is
only the label that is different). Use enzyme or antigen retrieval
as
usual. IF on formalin fixed skin and renal (like IPX) is difficult
but not impossible.
Collagen will fluoresce after formalin fixation and of course
melanin
will as well (basis of the formalin induced fluorescence test). The
appropriate use of filters will decrease this problem.
Regards, Tony
Tony Henwood
Senior Scientist
Anatomical Pathology
Royal Prince Alfred Hospital
Sydney, AUSTRALIA
http://www2.one.net.au/~henwood
http://www.pathsearch.com/homepages/TonyHenwood/default.html
----------------------------------------------------------------------
Date: 21 Oct 1999 10:00:48 -0500
From: "Jennings, M. Anita" <jennings@mayo.edu>
Subject: FW: FW: NSH Diary, Final
Last one for this year. Thanks a bunch Andi. Bring back all of your
newly
acquired knowledge and share with the rest of us. anita
OK Anita, one more entry for NSH 1999:
It was a dark and rainy nite...well it was! Cold too.
Workshops are over, and all the signs of NSH gone from the
convention
center.
It always seems sad to have witnessed all the excitement of a few
days ago
gone for another year. Now everyone is looking forward to going home
after
such a great week of educational opportunities taking with them all
the
things they learned from their colleagues.
The banquet was well attended and I can't remember the all award
recipients
but I couldn't help but feel as the accomplishments of each
recipient was
read off that these people are extremely deserving of their
recognition by
NSH. One award I do remember was a special award to Linda and Herb
for
giving
us Histonet! Congratulations Linda and Herb!!!
I don't know who won time contest tonight. =;-)
Tomorrow is the HOD Meeting - the society at work.
This has been a memorable week, but Anita, I hope that you make it
to
Wisconsin next year so you can continue on the NSH Diary tradition.
I'm
sending a histonet button home for you.
Over and out.
Andi
----------------------------------------------------------------------
Date: 21 Oct 1999 10:30:52 -0500
From: "Sebree Linda A." <la.sebree@hosp.wisc.edu>
Subject: RE: antibody to EBV antigen LMP-2
We use Cell Marque's LMP (latent membrame protein) cocktail which
consists
of 4 clones: CS1, CS2, CS3 and CS4. I wonder if by LMP-2, you are
referring to the CS2 clone? I don't know that I've seen these
clones as
separate antibodies but they probably are marketed as such
somewhere. Maybe
one of the vendors out there can tell you.
Linda A. Sebree, HT
University of Wisconsin Hospital & Clinics
Immunohistochemistry/In Situ Hybridization Laboratory
D4/218-2472
600 Highland Avenue
Madison, WI 53792-2472
(608)265-6596
FAX: (608)263-1568
> -----Original Message-----
> From: Sarka Lhotak [SMTP:lhotaks@fhs.csu.mcmaster.ca]
> Sent: Wednesday, October 20, 1999 7:33 PM
> To: histonet@pathology.swmed.edu
> Subject: antibody to EBV antigen LMP-2
>
> Hello all,
> Has anybody come across an antibody to the EBV antigen
LMP-2? Any
> lead would be greatly appreciated. Thanks,
>
> Sarka Lhotak
>
> Hamilton Regional Cancer Centre
> McMaster University
> Hamilton, Ontario
>
> lhotaks@mcmaster.ca
>
----------------------------------------------------------------------
Date: 21 Oct 1999 11:45:34 -0500
From: alexis <alexis.a.schulman.1@nd.edu>
Subject: Hypoxia in mouse tissue
Hi, all!
I am looking for a good antibody to visualise hypoxic cells in
paraffin
embedded mouse tumors. So far I've only found HIF-1alpha from
Labvision
but it hasn't been tested for immunos. I also found a reference to
NITP
(2- nitroimidazole theophyllin) developed at Brunel University, UK.
but no
mention to vendors or how to obtain it.
I would really appreciate any help!
Alexis
Center for Transgene Research
and the
Department of Chemistry and Biochemistry
University of Notre Dame
Notre Dame, IN, USA
46556
219-631-3539 (phone)
219-631-4048 (fax)
----------------------------------------------------------------------
Date: 21 Oct 1999 11:45:58 -0500
From: Jo-Ann Bader <jo-ann@lan1.molonc.mcgill.ca>
Subject: MITOCHONDRIAL STAIN
Hi all ,
I am looking for a stain specifically for mitocondria on paraffin
sections.
Is there such a thing?
Thanks in advance
Jo-Ann
----------------------------------------------------------------------
Date: 21 Oct 1999 12:46:00 -0500
From: "J. A. Kiernan" <jkiernan@julian.uwo.ca>
Subject: Re: MITOCHONDRIAL STAIN
On Thu, 21 Oct 1999, Jo-Ann Bader wrote:
> I am looking for a stain specifically for mitocondria on paraffin
sections.
> Is there such a thing?
Yes indeed. Mitochondria were discovered by staining them
in paraffin sections, and methods are given even in modern
techniques books. I haven't tried all the methods, but have
seen mitochondria using Baker's acid-haematein (a histochemical
method for choline-containing phospholipids) and with Bensley's
copper-haematoxylin, a traditional method. More ordinary dyes
will also stain mitochondria, but not selectively enough to
see them properly. The most colourful selective method is
said to be Altmann's aniline-acid fuchsine-picric acid, but
I've never tried that one.
Mitochondria can be stained only if they have been properly
fixed before embedding. A mitochondrion is mostly membrane, so
it's necessary to make the phospholipids insoluble before
dehydration, clearing and embedding. This can be done by a
treatment with osmium tetroxide or potassium dichromate (or
both) after primary fixation in neutral buffered formaldehyde,
with or without added glutaraldehyde. Helly's fluid also
preserves mitochondria. The traditional fixatives for cytoplasm
and organelles are unbuffered mixtures of OsO4 with K2Cr2O7,
used on very tiny pieces of tissue. The sections need to be
thin - 4 mu or less.
The selective stains for mitochondria also colour red blood
cells and myelin sheaths, but these do not cause confusion.
It's good to hear that there's still a demand for traditional
staining methods for mitochondria.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
E-mail: kiernan@uwo.ca
----------------------------------------------------------------------
Date: 21 Oct 1999 13:45:59 -0500
From: mvaicaitis@lcmh.org (Marija Vaicaitis)
Subject: rapid AE1/AE3
We routinely do an AE1/AE3 on all sentinel nodes. But now the
question came
up-if there is a possibility of doing a rapid
AE1/AE3 on frozen section slides. I would appreciate any
information!
Marija Vaicaitis
Pathology Lab
Little Co. of Mary Hospital
Evergreen Park, Il
----------------------------------------------------------------------
Date: 21 Oct 1999 14:00:37 -0500
From: "Kellar, Eric" <kellarec@MSX.UPMC.EDU>
Subject: RE: MITOCHONDRIAL STAIN
The mitochondrion was identified at the end of the 19th century by
Altmann.
Benda in 1903 coined the term mitochondrion, from mito, thread, and
khondrion, granule, because these organelles appeared as
thread-forming
granules under the light microscope. Only a few years later, most of
the
relevant functions of mitochondria, such as energy production, cell
respiration and inheritance, were suggested by Michaelis, Regaud and
Kingsbury. Even the biological origin of mitochondria as a symbiotic
fusion
between a prokaryote and a primitive eukaryote cell was hypothesized
by
Portier decades before it could be more firmly established.
The next development in mitochondrial staining after Altmann in
1890, was
the technique developed by Kull in 1914 using the highly explosive
dye,
aurantia. Kull also substituted the fixative of Champy 1913 for the
fixative specified by Altmann in 1890. On the basis that Champy
invented
this fixative for another purpose, the technique has been commonly
known as
the Champy-Kull technique. (any technique in which acid fuchsin is
used as
the primary stain). The two techniques of Champy-Kull and Parat (in
which
sections are "pre-dichromated") were brought together in 1928 by
Volkonsky.
The more modern and commonly used techniques are given by Cain (AFIP
Manual)
and another by Takaya, both of which treat the sections in Regaud's
solution
prior to staining.
I have tried both of these methods (preferring that of Cain) and
would be
glad to fax you a copy of either.
Eric C. Kellar
Histology/Immunohistochemistry
University of Pittsburgh Medical Center
----------
From: Jo-Ann Bader[SMTP:jo-ann@lan1.molonc.mcgill.ca]
Sent: Thursday, October 21, 1999 12:27 PM
To: HistoNet@Pathology.swmed.edu
Subject: MITOCHONDRIAL STAIN
Hi all ,
I am looking for a stain specifically for mitocondria on
paraffin
sections.
Is there such a thing?
Thanks in advance
Jo-Ann
----------------------------------------------------------------------
Date: 21 Oct 1999 14:23:41 -0500
From: dave@biocare.net
Subject: CD31 and CD10
Could some tell me a good CD31 and CD10 antibody for formalin-fixed
paraffin embedded tissues. What procedures and pretreatments are
you
using.
Dave
----------------------------------------------------------------------
Date: 21 Oct 1999 14:25:18 -0500
From: Atoska Gentry <gentras@vetmed.auburn.edu>
Subject: cryostat
Histonetters, especially those of you who replied to my previous
inquiry.
you who recommend the Hacker/Bright cryostat which models do you
have in
use and is your lab setting one of clinical or research? After a
little
research I've learned that Hacker manufactures a wide range or
cryostats
and I'm trying to decide which one will best suit our needs. Also
I'd like
to know your likes and dislikes in reference to your experience with
them.
you can email me personally if you do not wish to do so on the
histonet.
Thanks, Atoska
Atoska S. Gentry B.S., HT(ASCP)
Research Assistant
Scott-Rtichey Research Center
College of Veterinary Medicine
Auburn University, AL 36849
PH (334) 844-5579
FAX (334) 844-5850
----------------------------------------------------------------------
Date: 21 Oct 1999 15:21:51 -0500
From: "pathanjaly hariharan" <saradanjaly@yahoo.com>
Subject: CD31 and CD10
Hello Dave,
InnoGenex carries an anti-CD31(Clone 9G11) which is
validated for paraffin sections, as well as frozen.
For paraffin embedded tissues: Antigen Retrieval
Solution AR10(pH10) is recommended.
Primary incubation = 2 hours at 37 degrees C.
The data sheet can be accessed at:
http://www.innogenex.com
InnoGenex: (877)IGX INFO
If you would like more information, please give me a
call.
Best Regards,
Matthew Ogdie
InnoGenex
(925)543-1414
Could some tell me a good CD31 and CD10 antibody for
formalin-fixed
paraffin embedded tissues. What procedures and
pretreatments are you
using.
Dave
=====
__________________________________________________
Do You Yahoo!?
Bid and sell for free at http://auctions.yahoo.com
----------------------------------------------------------------------
Date: 21 Oct 1999 16:01:14 -0500
From: "Qiu, Yu-Shan" <yqiu@shctampa.usf.edu>
Subject: Manual for Polycut S
Can anyone in the histoland kindly send me a copy of Operating
Manual
for Polycut S? Due to the lab moving and people change, we lost ours
and
now in need to use this machine to finish our projects. I would
greatly
appreciate your help!
Yu-Shan Qiu
Dept. of research
Shriners Hospital for Children
Tampa, FL 33617
Tel. (813)972-2250 Ext.7582
Fax (813)975-7127
----------------------------------------------------------------------
Date: 21 Oct 1999 17:30:29 -0500
From: Histonet <Histonet@dakousa.com>
Subject: RE: CD31 and CD10
Dear Dave,
DAKO Corporation sells a CD31, clone JC/70A (M0823), that can be
used on
FFPE sections. We recommend enzymatic digestion as a pretreatment.
Unfortunately, our CD10 can only be used on frozen sections. Please
contact
Technical Services if you would like further information.
Sincerely,
Danielle McCombs
DAKO Corporation
Technical Service Specialist
email danielle.mccombs@dakousa.com
(800)235-5743 ext. 5321
- -----Original Message-----
From: Dave Tacha [mailto:dtacha@ncal.verio.com]
Sent: Thursday, October 21, 1999 11:50 AM
To: histonet@Pathology.swmed.edu
Subject: CD31 and CD10
Could some tell me a good CD31 and CD10 antibody for formalin-fixed
paraffin embedded tissues. What procedures and pretreatments are
you
using.
Dave
----------------------------------------------------------------------
Date: 21 Oct 1999 18:45:25 -0500
From: "Vicki L McKaughan" <vlm_23@n2mail.com>
Subject: immunostainer
I am interested in purchasing an automated immunostainer.
currently, I am
demoing the
Ventana. I am schduled to demo the biogenics
and dako stainers also. i would appreciated hearing from anyone who
currently
uses one of these instruments.
sincerely,
vicki mckaughan, HT
What are you N2? Choose from 150 free e-mail addresses.
http://www.n2mail.com
----------------------------------------------------------------------
Date: 21 Oct 1999 19:30:17 -0500
From: RhodaRocks@aol.com
Subject: Glycerin Antigen Retrieval Solution
A little while ago someone mentioned an antigen retrieval method
using a
Glycerin solution for ER-PR. Could you please mention the specifics
one more
time? Thanks
----------------------------------------------------------------------
Date: 21 Oct 1999 22:15:35 -0500
From: ODDBALSTER@aol.com
Subject: Fetus/POC Policy
Hello Histonetters,
I have an interesting story/question. Twice this year
I have
been asked by the family of a patient who had fetal demise
(delivered or
c/s), at 19wks to view the fetus. Now, I would normally have no
objections
(even though I feel it isn't something wonderful to see for the
grieving
family) but in both of these instances, we received the
fetus/placenta
already in 10% Zinc Formalin (they have Formalin in the OB unit and
bring the
specimens to the lab already submerged). Does anyone out there have
or
implemented a policy regarding this situation? My risk manager was
at a loss
as to what to come of the situation, so we tried to sway the
family's
descision (at no avail) and we ended up letting them "view" the
fetus through
the original container (frosted and impossible to see anything
clearly),
telling them they were unable to open the container due to the
hazardous
materials contained in it. This apparently was enough "closure" for
them and
they were satisfied. But my risk manager and I still want to make a
hospital
policy concerning this issue. I am in Florida and I was wondering if
state or
local laws regarding Formalin and patient/non-hospital employees
contact
would be an issue? OSHA maybe? We have a release form for fetus' to
funeral
homes only, but we never release Formalin contaminated
tissue/objects to a
patient. Has this happened to anyone else out there? What did you
do? I would
really appreciate any information you might have.
K. Zajic
Histology Supervisor
West Palm Beach, FL
Here are the messages received yesterday!
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