RE: Staining Cell Cultures

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From:"Bennett, Catherine (Katie)" <> (by way of histonet)
Date:Wed, 20 Oct 1999 03:46:46 -0400
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I would recommend that you tell your client that he should grow the cells
directly onto a slide using cell culture chamber slides.  These special
slides have a little chamber that is attached directly onto the slide,
allowing you to culture your cells right on the slide.  Once you are done,
you just pop off the well walls and are left with the cells on the slide.
Nalge makes a whole line of them and you should be able to find them in any
VWR or Fisher catalog.  In a study I was involved in where we wanted to
stain for actin filaments, we grew the cells up right on the slide until
~90% confluent, fixed the cells, then did the immunohistochemistry staining
right in the cell well chambers.  We only removed the well wall after
staining was complete, then coverslipped and analyzed on the scope.

Catherine "Katie" Bresee Bennett
Sr. Technical Associate
Lovelace Respiratory Research Institute
Albuquerque, New Mexico

> -----Original Message-----
> From:	HistoScientific []
> Sent:	Tuesday, October 19, 1999 8:08 AM
> To:
> Subject:	Staining Cell Cultures
> Dear Netters,
> We have a client that would like to see lipid vesicles in cell cultures
> that he has developed in a Petri dish.  How do we get the cell culture
> on the microscope slide?  Is the culture something you can frozen
> section, then stain?  We work with the actual tissue most of the time,
> therefore we are not very experienced with cell cultures!  Any
> suggestions would be most appreciated.
> Sincerely,
> Beth Poole
> Histo-Scientific Research Labs.
> 107 Killmon Road
> P.O. Box 30
> Basye, VA  22810
> (540)856-2222
> fax: (540)856-2227

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