Lipids In Barnacle Cyprids!!(HELP)

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From:Bruce Abaloz <b.abaloz@zoology.unimelb.edu.au> (by way of histonet)
To:histonet@histosearch.com
Reply-To:
Date:Mon, 31 Jan 2000 22:47:13 -0500
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Dear Histonneters, We have a student doing her PhD studying CYPRIDS. A Cyprid
is the free swimming stage of the Sea - Water Barnacle before it settles
permanently! Our student Marsha would like to look @ the lipid content/volume
& also the protein content/volume of individual Cyprids,ALSO looking @ the
nervous system Histologically to see if it changes morphologically as they
age/mature in the Cyprid stage.....as well as the  newly settled Barnacle
stage. What fixative would be utilized & is it possible to demonstrate both
lipids & proteins with the same procedure/method etc.? Marsha has access to
a Confocal
Microscope & is hopeful that someone can suggest a method/s to look @ the
specimen under the Confocal to determine amounts of protein/Lipid ??) ANY
help/suggestions will be GREATLY appreciated.......................
Cheers, Bruce (& Marsha) in OZ.


--
BRUCE ABALOZ
HISTOLOGIST
DEPARTMENT of ZOOLOGY	         *  ph:    +61 3 93446282
The UNIVERSITY of MELBOURNE      *  fax:   +61 3 93447909
Parkville Victoria.3052          *  email: b.abaloz@zoology.unimelb.edu.au

AUSTRALIA.
                    IF YOU WANT TO BE HAPPY......BE!!
                    I DO NOT SEEK............I FIND!!

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