In Situ with Bone

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From:Jay Turner <> (by way of histonet)
Date:Wed, 20 Oct 1999 03:46:53 -0400
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Does any one have any suggestions for a time-efficient in situ
hybridization protocol for bone specimens?  I was hoping to avoid
the long, painful process of EDTA decalcification, if possible.
Would a dilute formic acid decal solution be gentle enough for mRNA
preservation?  Is there an embedding medium for nondecalcified bone
that is suitable for in situ?  Any help would be greatly


Jay D. Turner
Dept. of Orthopaedics
University of Medicine and Dentistry of NJ
Newark, NJ

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