Immuno after H&E on a in-situ slide

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From:Cynthia A Delong <DELONG_CYNTHIA_A@LILLY.COM> (by way of histonet)
Date:Mon, 31 Jan 2000 22:47:29 -0500
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Hello everyone,

I have a question regarding doing  immuno on a in-situ after it has been
stained with H & E.   Does anyone have any sort of protocol for this.

I believe after removing the coverslip I would run it backwards to water and
then start my procedure right????  Anyway if anyone has such a thing would you
please forward it to me.   Do you do antigen retrieval on this section.  What
damage if any will the in-situ have.  Note: It has been labeled with a
radioactive probe.

Thanks for all of your help
Fax:  317-277-6146

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