Immuno after H&E on a in-situ slide

From:	Cynthia A Delong <DELONG_CYNTHIA_A@LILLY.COM> (by way of histonet)
To:	histonet@histosearch.com
Reply-To:
Date:	Mon, 31 Jan 2000 22:47:29 -0500
Content-Type:	text/plain; charset="us-ascii"



Hello everyone,

I have a question regarding doing  immuno on a in-situ after it has been
stained with H & E.   Does anyone have any sort of protocol for this.

I believe after removing the coverslip I would run it backwards to water and
then start my procedure right????  Anyway if anyone has such a thing would you
please forward it to me.   Do you do antigen retrieval on this section.  What
damage if any will the in-situ have.  Note: It has been labeled with a
radioactive probe.

Thanks for all of your help
Cindy
317-276-7635
Fax:  317-277-6146

<< Previous Message | Next Message >>