Hi Everyone!

One of our investigators would like to do a study with
this antibody on rats/mice brain tissue.
I have read several articles in reference to it being
the most widely used marker for the evaluation of
oxidative DNA damage.

However, I have a need for control tissue.  Since it
is known to stain kidney, I thought we should use an
untreated kidney and one that has been treated with
Ferric Nitrilotriacetate (basis of the study).  But I
also wanted to see if anyone else had used this
antibody and how they handled their controls.

This particular antibody seems to have some have cross
reactivity with 8-hydroxyguanosine (8-OHG).  One
notation I read said that the cross reaction with
guanine and guanosine occurs at 20,000 fold lower than
for 8-hydroxyguanine.  It will also cross react with
structurally related 8-hydroxyguanine derivatives like
5-hydroxymethyl-uridine but with a much lower binding
affinity.

My quieries are:

Has anyone used this particular antibody?
monoclonal? (possibly N45.1)
polyclonal?
Have you seen this cross reactivity in your studies?
Were you able to reproduce your results.
What control tissue did you use?
Did you use avidin in a parallel run as well?

Any help would be appreciated.

Thanks

Vikki Baker
American Health Foundation - Valhalla, New York
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