need to preserve oocytes inside follicle

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From:"Kathy Oprea" <>
Date:Mon, 11 Oct 1999 13:39:04 EST
Content-Type:text/plain; charset=US-ASCII

Hi all, 
We have a researcher that has submitted Bovine ovarian cortex fixed 
in formalin. He needs to see the oocyte inside the antral follicle. 
Does anyone know the best way to process, cut and stain the follicle 
to prevent shrikage and preserve the integrity of the follicles so 
that the oocyte may be oserved? The tissue is fixed less than 12 
hours. He has tried Zenkers and Bouins fixed tissue but has  not been 
successful yet. Any suggestons?
Thank you,
************ Thank you ************
 Kathy Oprea                     
 University of Georgia          
 College of Veterinary Medicine  
 Athens, GA 30602-7388            
The worker who's never said "Thank
God it's Friday," probably never
really worked.

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