Re: cells from culture

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From:Kathy Wortham <>
To:Jill Songer <>
Date:Wed, 13 Oct 1999 06:54:41 -0700 (PDT)
Content-Type:text/plain; charset=us-ascii

The agar will do fine in the processor, cells should
remain intact in the block of agar so embbedding will
not be a problem. Depending on the protein content of
the agar, background might be a problem but the agar
should not affect the label.

--- Jill Songer <> wrote:
> Hi Histonetters,
> Before everyone, except me, heads off to NSH, let me
> pick your brains. We
> have a researcher who has brain cells grown from
> cell cultures. These cells
> are in agar. Sorry, don't know what kind. He is
> interested in doing
> immunocytochemistry on the cells and wonders if the
> agar will interfere
> with the immuno label. Again, I don't know what
> label. Also, any tips on
> processing these little suckers and how to embed
> them without losing them.
> Will the agar stay put during processing or will it
> dissolve?
> Thanks.
> Jill Songer HT (ASCP)
> Virginia-Maryland Regional College of Veterinary
> Medicine
> Veterinary Teaching Hospital
> Virginia Tech
> "To achieve the possible, we must attempt the
> impossible"

Kathy Wortham
USDA,Food Safety Inspection Service
950 College Station Road
Athens, Ga 30605
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