Re: Xylene Subs

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From:Robert Santoianni <>
Date:Fri, 15 Oct 1999 08:36:12 -0500
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We've used Richard-Allan Scientific Clear Rite III for processing with
great results.  Longer times are necessary than with xylene for clearing
but Clear Rite is less harsh on tissue and we've experienced better
sectioning characteristics and less brittleness with potientially
difficult specimens such as liver, etc.  Stick with xylene for dewaxing
in your purge (cleaning) cycle for your processors and for staining.  If
you are coverslipping by hand, you can go directly into Clear Rite from
your staining run.  Holding slides in Clear Rite is less noxious than
xylene, but you should have adequate downdraft fume extraction just the
same.  Recycling is no problem.  We've been doing it for almost 10
years!  Don't even think about limonene based products!
Bob Santoianni
Emory University Hospital
Atlanta, GA

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