Re: Thomas Histology (metanil yellow)
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> |
To: | Histonet <histonet@pathology.swmed.edu> |
Reply-To: | |
Date: | Thu, 7 Oct 1999 01:33:27 -0400 (EDT) |
Content-Type: | TEXT/PLAIN; charset=US-ASCII |
On Thu, 7 Oct 1999, Ken Turner wrote:
> I have a copy of Thompson, which, on page 480 gives a metanil yellow
> counterstain after PAS. The solution is: 0.25% metanil yellow in 0.25%
> acetic acid prepared with distilled water. Mix, filter and store in a clean
> brown bottle.
> After conventional periodic acid and Schiff sequence, and water washing,
> stain 1 minute in metanil yellow, rinse in distilled water, 95% ethyl
> alcohol, absolute ethyl alcohol and xylene. Thompson suggests mounting with
> Permount.
For what it's worth, I agree 100%. Metanil yellow provides
a splendid yellow counterstain for cytoplasm and collagen.
Unfortunately the dye solution is not very stable. Its colour
changes to brown over the course of a few months, and the
staining properties also deteriorate. I don't know why, but
it's so. For another yellow anionic dye you might try
tertrazine. Look it up in Bryan Llewellyn's StainsFile: a
splendid collection of histo-wisdom.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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